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UNIFILTER Microplate, 96well, 800 µl, DNA binding, clear polystyrene UNIFILTER 96well microplate, 800 µl, long drip director No cross talk. Patented integral filter design prevents welltowell cross contamination. Economical to use. Wide range of well volume options ensures efficient use of materials. Better control. Choice of filter media allows control of the flow rates and retention characteristics. Versatile. A broad range of filtration media is available, including glass fiber, polypropylene, cellulose nitrate, cellulose acetate, nylon, and ion exchange cellulose. DNA recovery of 6 ?g per well on average. Consistent yield across all 96 wells Eluted plasmid DNA is free of genomic DNA contamination when used with Lysate Clarification UNIFILTER. Highquality DNA suitable for PCR, restriction digestion, and sequencing. Save time: No desalting or ethanol precipitation. No kit required; significantly reduces costs. The 800 µl UNIFILTER microplate is most typically used in purifications, isolations, and separations of biomolecules, particularly DNA. The microplate has a well volume of 800 ?l, which is excellent for standard DNA plasmid miniprep chemistries. The choice of short or long drip directors is applicationspecific. UNIFILTER 800 ?l is constructed from rigid highgrade polystyrene. Proprietary Whatman UNIFILTER microplates with filter bottom wells are convenient and ready to use. Available in 24, 96, and 384well formats, UNIFILTER microplates offer a choice of filter media to meet exact application requirements. The novel drip director design of Whatman UNIFILTER microplates ensures precise collection of the filtrate to allow for further processing and analysis. UNIFILTER microplates are available in a range of well volumes from 100 ?l to 10 ml. Plasmid DNA Binding UNIFILTER works either as a standalone or as part of our highthroughput miniprep system. Plasmid DNA is bound to the filter under chaotropic conditions, washed twice, and then vacuumed to dryness on a vacuum manifold. The plasmid DNA is eluted by vacuum in a final volume of 100 ?l into a nonbinding polypropylene collection plate using water or TEą buffer. The DNA is ready to use and further ethanol precipitation is unnecessary. The final concentration is 50 to 100 ng/?l, depending on the original culture. The A260/280 ratio is 1.9 and the yields in all 96 wells “max out” at 6 ?g. The Plasmid DNA binding plate can be used with both vacuum and centrifuge techniques, making it a vital and flexible tool in every highthroughput laboratory.
